FastQCFastQC Report
Wed 4 May 2016
G131-M2_CGATGT_BC8GTYANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M2_CGATGT_BC8GTYANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13306075
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG358430.2693731998354135Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG53450.032.54640634
GTAGATC54200.031.97635731
ATCTCGG55300.031.33805835
TCGTGTA55000.030.80095114
GCGTCGT56800.030.44632311
TCTCGGT57450.029.97397436
CGTCGTG59200.029.2860112
CGTGTAG58300.029.1663215
AGCGTCG60600.028.93739510
GTAGGGA59350.028.722518
GTCGCCG61100.028.4398444
GAGTGTA62100.028.0199327
GTCGTGT61350.027.97257613
GGTCGCC64250.027.14607243
TGGTCGC63900.027.0880442
AGTGTAG64450.027.06407428
GAGCGTC65000.026.776169
TAGATCT65300.026.67569232
GATCGGA64300.026.543331
TAGGGAA63500.026.5336119
Produced by FastQC (version 0.11.2)