FastQCFastQC Report
Wed 4 May 2016
G131-M4_ATGTCA_BC8GTYANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M4_ATGTCA_BC8GTYANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14629393
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1058440.7235023353327099Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234400.1602253764048857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG133150.038.039234
TCTCGGT134000.037.78136436
GTAGATC135200.037.67685731
TCGTGTA134850.037.11583314
ATCTCGG136650.037.04830635
GTCGCCG139350.036.27342644
GCGTCGT139900.036.24931711
TGGTCGC140550.036.1012442
CGTGTAG139650.035.97757315
GTAGGGA141050.035.78945518
CGTCGTG141950.035.69457212
GGTCGCC143050.035.4704443
GAGTGTA143400.035.4208527
GTCGTGT143500.035.26290513
AGCGTCG146500.035.1430710
AGTGTAG145350.034.98829328
TAGATCT145400.034.98755632
TGTAGGG146200.034.7249317
CTCGGTG146650.034.7024337
GTGGTCG146700.034.64776641
Produced by FastQC (version 0.11.2)