FastQCFastQC Report
Wed 4 May 2016
G131-M5_CCGTCC_BC8GTYANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M5_CCGTCC_BC8GTYANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11083013
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC292740.26413394985641536TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA51400.039.192881
CGTATGC50500.038.08423644
ACCCGTC52150.037.75942232
TCGTATG50700.037.63154643
ATCGGAA54050.037.060382
ACACGTC55750.035.5940113
CGTCTGA56350.035.2094116
ACGTCTG57300.034.94610215
CACCCGT59300.033.54428531
TCACCCG59800.033.40766530
CACGTCT60750.032.88794314
CTCGTAT56250.032.82560742
CACACGT61900.032.35804712
CGGAAGA65250.031.0391714
TCTCGTA54500.030.73395541
TCGGAAG66900.030.5983873
CCCGTCC66400.029.7001733
CCGTCCA66200.029.6348334
CGTCCAT59550.029.39821835
GCACACG68500.029.24113311
Produced by FastQC (version 0.11.2)