FastQCFastQC Report
Wed 4 May 2016
G131-M5_CCGTCC_BC8GTYANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M5_CCGTCC_BC8GTYANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11083013
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG398590.3596404696087607Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC61250.034.68848831
TCTCGGT62300.034.52422336
GATCTCG62450.034.16003434
TCGTGTA61900.033.76501514
ATCTCGG63900.033.55636235
GCGTCGT65300.032.58104311
CGTGTAG66050.031.8063315
CGTCGTG67500.031.45381212
AGCGTCG68900.031.39074710
TAGATCT68700.031.21378732
GTAGGGA68150.031.05050318
TGGTCGC68950.030.97316442
GTCGTGT68150.030.95968413
GTCGCCG68700.030.896344
GAGTGTA68950.030.81965827
AGTGTAG69050.030.70867228
AGAGCGT70250.030.4468428
ATCGGAA69950.029.876842
GGTCGCC71400.029.7256743
TAGGGAA71350.029.5654219
Produced by FastQC (version 0.11.2)