FastQCFastQC Report
Wed 4 May 2016
G131-M6_GTCCGC_BC8GTYANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M6_GTCCGC_BC8GTYANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12287457
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG628830.5117657787123894Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC81200.036.7032831
GATCTCG81300.036.4656434
TCGTGTA78900.036.3612714
ATCTCGG81750.036.18361735
TCTCGGT82600.035.8915836
GCGTCGT83950.034.9614611
CGTCGTG86600.033.94257412
CGTGTAG85750.033.8363615
GTCGTGT86550.033.60574313
AGCGTCG89250.033.42833710
GAGTGTA89150.033.23802627
GTCGCCG89350.033.1357644
TAGATCT89900.033.10134532
AGTGTAG90200.033.06925228
GATCGGA89800.032.981241
GTAGGGA88700.032.8330218
TGGTCGC91750.032.43469642
ATCGGAA91150.032.4202882
AGAGCGT92650.032.1339048
GAGCGTC93650.031.9522519
Produced by FastQC (version 0.11.2)