FastQCFastQC Report
Wed 4 May 2016
G131-M7_GTGAAA_BC8GTYANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M7_GTGAAA_BC8GTYANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14852273
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA896350.6035103179156484No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT632800.42606273127352295TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA102200.041.0124661
TGAAACG101350.039.18835435
ATCGGAA106650.039.1494982
ACACGTC106900.039.10648713
ACGTCTG109900.038.4228915
GTCACGT107650.038.17291629
AACGATC86350.038.13136338
CGTCTGA110800.038.03825416
AGTCACG107950.037.68206828
CTCGTAT99700.037.63372444
GAAACGA105450.037.43475736
AAACGAT93950.037.2247537
CACGTGA109950.036.88068431
TCACGTG112250.036.72826430
CACGTCT116000.036.3239614
CGGAAGA116000.036.190094
ACGATCT91700.036.12190639
CACACGT115700.035.91732812
TCGGAAG120450.035.1035353
TCTCGTA98700.033.87381443
Produced by FastQC (version 0.11.2)