FastQCFastQC Report
Wed 4 May 2016
G131-M8_GTGGCC_BC8GTYANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M8_GTGGCC_BC8GTYANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19778183
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT474800.24006249714647704TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA77350.037.8846971
ATCGGAA82700.035.26872
ACACGTC82550.034.52385313
ACGTCTG84950.034.12484415
CTCGTAT77700.033.80451644
GTCACGT85500.033.68453229
CGTCTGA85850.033.5863816
ACGTGGC86100.033.1792732
AGTCACG85950.033.1635828
TATCTCG75850.031.90812941
CACGTCT94000.030.76653914
TCACGTG95200.030.30933430
CGGAAGA97750.030.2934784
CACACGT94600.030.26971612
CGTGGCC93850.030.16962433
TCGGAAG102450.029.114543
TCTCGTA83850.028.8096443
GCACACG102850.028.05694811
GCCTTAT91800.027.32829937
CACGTGG108250.026.67821331
Produced by FastQC (version 0.11.2)