FastQCFastQC Report
Wed 4 May 2016
G131-M9_GTTTCG_BC8GTYANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M9_GTTTCG_BC8GTYANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11455570
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT476480.41593739988494677TruSeq Adapter, Index 21 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA223640.195223808156207No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218690.1909027660779865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA70350.040.6566621
CGTTTCG70200.040.42917633
ACGTTTC73650.038.64105232
ATCGGAA74000.038.5275082
ACACGTC75650.037.7576413
ACGTCTG75850.037.60528615
CTCGTAT70200.037.51004844
AGTCACG75450.037.4292428
CGTCTGA77250.036.78105516
CGGAATC65250.036.69349738
TCGGAAT68100.036.48446337
GTCACGT78400.036.37053329
TTTCGGA77950.035.8626435
GTTTCGG79450.035.68323534
CACACGT80550.035.46372212
AATCTCG68000.035.39675541
CGGAAGA81900.035.137464
TTCGGAA80100.034.59738536
CACGTCT83450.034.5478814
TCTCGTA71250.033.71898743
Produced by FastQC (version 0.11.2)