FastQCFastQC Report
Tue 17 May 2016
G128-M11-B_GGCTAC_AC8WFRANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M11-B_GGCTAC_AC8WFRANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11126814
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3599063.234582693662355Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA304540.27369919188008357No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176210.15836518881325778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA470150.043.194551
TCGTGTA474350.043.0242814
ATCGGAA469200.042.9397282
AGAGCGT482050.042.9116638
GTCGTGT491250.042.8383513
TCTCGGT478100.042.8206436
CGTGTAG475300.042.81794415
GATCTCG473700.042.6982434
ATCTCGG473700.042.63786335
GTGGTCG468650.042.56331341
GTAGATC478000.042.56151231
CGTCGTG490450.042.54938512
AAGAGCG476350.042.404717
GTAGGGA460900.042.3612318
TCGGTGG489200.042.3360438
CGGTGGT485350.042.2845239
TGTAGGG488700.042.2292817
CTCGGTG478900.042.21623637
GGTCGCC462950.042.2068743
GCGTCGT495900.042.205611