Basic Statistics
Measure | Value |
---|---|
Filename | G128-M11-B_GGCTAC_AC8WFRANXX_L001_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11126814 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 359906 | 3.234582693662355 | Illumina Single End PCR Primer 1 (100% over 50bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 30454 | 0.27369919188008357 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 17621 | 0.15836518881325778 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 47015 | 0.0 | 43.19455 | 1 |
TCGTGTA | 47435 | 0.0 | 43.02428 | 14 |
ATCGGAA | 46920 | 0.0 | 42.939728 | 2 |
AGAGCGT | 48205 | 0.0 | 42.911663 | 8 |
GTCGTGT | 49125 | 0.0 | 42.83835 | 13 |
TCTCGGT | 47810 | 0.0 | 42.82064 | 36 |
CGTGTAG | 47530 | 0.0 | 42.817944 | 15 |
GATCTCG | 47370 | 0.0 | 42.69824 | 34 |
ATCTCGG | 47370 | 0.0 | 42.637863 | 35 |
GTGGTCG | 46865 | 0.0 | 42.563313 | 41 |
GTAGATC | 47800 | 0.0 | 42.561512 | 31 |
CGTCGTG | 49045 | 0.0 | 42.549385 | 12 |
AAGAGCG | 47635 | 0.0 | 42.40471 | 7 |
GTAGGGA | 46090 | 0.0 | 42.36123 | 18 |
TCGGTGG | 48920 | 0.0 | 42.33604 | 38 |
CGGTGGT | 48535 | 0.0 | 42.28452 | 39 |
TGTAGGG | 48870 | 0.0 | 42.22928 | 17 |
CTCGGTG | 47890 | 0.0 | 42.216236 | 37 |
GGTCGCC | 46295 | 0.0 | 42.20687 | 43 |
GCGTCGT | 49590 | 0.0 | 42.2056 | 11 |