FastQCFastQC Report
Tue 17 May 2016
G128-M12-B_CTTGTA_AC8WFRANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M12-B_CTTGTA_AC8WFRANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21116602
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1248550.5912646362326666Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG175150.039.89100615
CGTCGTG179100.039.67460312
GCGTCGT179500.039.65954211
GATCGGA176200.039.5295641
TCGTGTA178400.039.3986814
TCTCGGT177400.039.2986336
GATCTCG175700.039.14046534
GTCGTGT186800.038.55737313
GTAGATC179250.038.41293331
AGAGCGT184600.038.3493428
ATCTCGG180900.038.1977835
ATCGGAA181100.038.1441732
GTAGGGA176550.037.9797318
GTGTAGG187900.037.5237216
TGTAGGG188900.037.38330517
AGCGTCG189100.037.33204710
GTGGTCG184850.037.20187441
GGTCGCC185500.036.6382343
AAGAGCG190900.036.48467
GAGCGTC194000.036.2870679
Produced by FastQC (version 0.11.2)