FastQCFastQC Report
Tue 17 May 2016
G128-M13-B_AGTTCC_AC8WFRANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M13-B_AGTTCC_AC8WFRANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16504883
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2234401.3537811809995866TruSeq Adapter, Index 14 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT952820.5772958220909533No Hit
GTCTTTTTGTTATTTTGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271890.16473306717775582No Hit
GGAGCATACTGGTGCTGATTATGAAGAGGAGTTCAAAGGGGGCGCTGTCT264470.16023742791754417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA279150.042.2798651
CTCGTAT266400.041.87637744
TCCGTAT259850.041.6867937
CGTCTGA277950.041.51216516
ACGTCTG278350.041.5078315
ACACGTC279300.041.20124413
CGTATCT260300.041.16692439
GTTCCGT276150.040.8209435
TATCTCG264150.040.61737441
ATCGGAA288800.040.607672
CACGTCT285600.040.4310414
CACACGT286000.040.3898812
CCGTATC264450.040.36295738
AGTTCCG283350.039.7839134
TCTCGTA271300.039.74964543
TTCCGTA283700.039.5947636
TCGGAAG299250.039.2043233
CGGAAGA300850.038.827654
GCACACG307150.038.28908511
GAACTCC311450.037.57691621
Produced by FastQC (version 0.11.2)