FastQCFastQC Report
Tue 17 May 2016
G128-M2-B_CGATGT_AC8WFRANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M2-B_CGATGT_AC8WFRANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12534690
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3952753.1534485495851907TruSeq Adapter, Index 2 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT624020.49783440994551925No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148110.11816008214004495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA472350.043.5871121
ATCGGAA474500.043.1397442
ACACGTC472450.042.69367213
CGTATGC472750.042.67242444
ACGTCTG473650.042.6598215
CGTCTGA472550.042.5729116
TCGGAAG480400.042.5091823
TCGTATG479050.042.4143343
CGATGTA470300.042.40942434
CCGATGT471350.042.40931733
CTCGTAT470250.042.3846842
CACGTCT476900.042.3460414
TCACCGA474300.042.34567330
CGGAAGA482150.042.1997644
ACCGATG475850.042.19849432
CACACGT479050.042.1743512
TATCTCG453600.042.16003439
CACCGAT476300.042.1447731
GTCACCG482650.042.01304229
TCTCGTA456950.041.9477441