Basic Statistics
Measure | Value |
---|---|
Filename | G128-M2-B_CGATGT_AC8WFRANXX_L001_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 12534690 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC | 395275 | 3.1534485495851907 | TruSeq Adapter, Index 2 (100% over 50bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 62402 | 0.49783440994551925 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 14811 | 0.11816008214004495 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 47235 | 0.0 | 43.587112 | 1 |
ATCGGAA | 47450 | 0.0 | 43.139744 | 2 |
ACACGTC | 47245 | 0.0 | 42.693672 | 13 |
CGTATGC | 47275 | 0.0 | 42.672424 | 44 |
ACGTCTG | 47365 | 0.0 | 42.65982 | 15 |
CGTCTGA | 47255 | 0.0 | 42.57291 | 16 |
TCGGAAG | 48040 | 0.0 | 42.509182 | 3 |
TCGTATG | 47905 | 0.0 | 42.41433 | 43 |
CGATGTA | 47030 | 0.0 | 42.409424 | 34 |
CCGATGT | 47135 | 0.0 | 42.409317 | 33 |
CTCGTAT | 47025 | 0.0 | 42.38468 | 42 |
CACGTCT | 47690 | 0.0 | 42.34604 | 14 |
TCACCGA | 47430 | 0.0 | 42.345673 | 30 |
CGGAAGA | 48215 | 0.0 | 42.199764 | 4 |
ACCGATG | 47585 | 0.0 | 42.198494 | 32 |
CACACGT | 47905 | 0.0 | 42.17435 | 12 |
TATCTCG | 45360 | 0.0 | 42.160034 | 39 |
CACCGAT | 47630 | 0.0 | 42.14477 | 31 |
GTCACCG | 48265 | 0.0 | 42.013042 | 29 |
TCTCGTA | 45695 | 0.0 | 41.94774 | 41 |