FastQCFastQC Report
Tue 17 May 2016
G128-M2-B_CGATGT_AC8WFRANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M2-B_CGATGT_AC8WFRANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12534690
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3332882.6589249514746673Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT805980.6429995476553468No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA252410.2013691603063179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA432650.043.1611441
AGAGCGT445450.043.0445638
TCGTGTA436950.043.01091814
ATCGGAA431850.042.9558142
CGTCGTG447800.042.87762512
GTCGTGT452100.042.86881313
CGTGTAG438700.042.7591115
GCGTCGT450750.042.65051711
GATCTCG436200.042.6119934
GTGGTCG434700.042.6096641
ATCTCGG436450.042.5976635
TCTCGGT442900.042.53362736
AAGAGCG441500.042.303577
GGTCGCC431500.042.28948643
GAGCGTC448700.042.2277959
CTCGGTG443150.042.1720637
TCGGTGG452100.042.1419538
GTAGATC442950.042.13377831
AGCGTCG451300.042.0818410
TCGGAAG443750.042.051763