Basic Statistics
Measure | Value |
---|---|
Filename | G128-M2-B_CGATGT_AC8WFRANXX_L001_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 12534690 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 333288 | 2.6589249514746673 | Illumina Single End PCR Primer 1 (100% over 50bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 80598 | 0.6429995476553468 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 25241 | 0.2013691603063179 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 43265 | 0.0 | 43.161144 | 1 |
AGAGCGT | 44545 | 0.0 | 43.044563 | 8 |
TCGTGTA | 43695 | 0.0 | 43.010918 | 14 |
ATCGGAA | 43185 | 0.0 | 42.955814 | 2 |
CGTCGTG | 44780 | 0.0 | 42.877625 | 12 |
GTCGTGT | 45210 | 0.0 | 42.868813 | 13 |
CGTGTAG | 43870 | 0.0 | 42.75911 | 15 |
GCGTCGT | 45075 | 0.0 | 42.650517 | 11 |
GATCTCG | 43620 | 0.0 | 42.61199 | 34 |
GTGGTCG | 43470 | 0.0 | 42.60966 | 41 |
ATCTCGG | 43645 | 0.0 | 42.59766 | 35 |
TCTCGGT | 44290 | 0.0 | 42.533627 | 36 |
AAGAGCG | 44150 | 0.0 | 42.30357 | 7 |
GGTCGCC | 43150 | 0.0 | 42.289486 | 43 |
GAGCGTC | 44870 | 0.0 | 42.227795 | 9 |
CTCGGTG | 44315 | 0.0 | 42.17206 | 37 |
TCGGTGG | 45210 | 0.0 | 42.14195 | 38 |
GTAGATC | 44295 | 0.0 | 42.133778 | 31 |
AGCGTCG | 45130 | 0.0 | 42.08184 | 10 |
TCGGAAG | 44375 | 0.0 | 42.05176 | 3 |