FastQCFastQC Report
Tue 17 May 2016
G128-M4-B_TGACCA_AC8WFRANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M4-B_TGACCA_AC8WFRANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9408758
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3860984.103602197016865TruSeq Adapter, Index 4 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT512470.5446733777189295No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA314830.33461377155199445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA469200.043.707211
CGTATGC469350.043.18781744
ATCGGAA472100.043.1816182
ACACGTC470450.043.00573713
ACGTCTG472650.042.93123215
TCGTATG475650.042.84242243
CGTCTGA471950.042.83642616
TCGGAAG475900.042.8090823
CTCGTAT459100.042.75761842
CGGAAGA474400.042.7079474
CACGTCT475050.042.6541414
CACACGT476050.042.6153712
TCTCGTA446050.042.55361641
ATCTCGT450500.042.11862640
ACTGACC481500.042.03006732
AATCTCG449550.041.9576139
GACCAAT461000.041.6994435
GAACTCC490900.041.67151321
ACCAATC449950.041.5347236
GCACACG495400.041.394911
Produced by FastQC (version 0.11.2)