FastQCFastQC Report
Tue 17 May 2016
G128-M4-B_TGACCA_AC8WFRANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M4-B_TGACCA_AC8WFRANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9408758
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3361123.5723312258642426Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1324811.4080604475107128No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA287180.3052262583435561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA435300.043.5193941
TCGTGTA437100.043.3740314
GTCGTGT452050.043.331413
AGAGCGT447750.043.329918
ATCGGAA434450.043.31592
CGTCGTG450500.043.0754112
GATCTCG436900.043.042134
CGTGTAG439550.043.0073715
GCGTCGT452700.042.9970511
ATCTCGG437550.042.95302235
AAGAGCG441950.042.9380877
GTGGTCG433750.042.8706241
GGTCGCC426050.042.8657943
TCTCGGT443400.042.8080536
TCGGAAG443100.042.728483
AGCGTCG451450.042.6190610
TCGGTGG449750.042.60098638
CTCGGTG441600.042.58399637
CGGTGGT446900.042.57016839
GAGCGTC451250.042.5697029