Basic Statistics
Measure | Value |
---|---|
Filename | G128-M4-B_TGACCA_AC8WFRANXX_L001_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9408758 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 336112 | 3.5723312258642426 | Illumina Single End PCR Primer 1 (100% over 50bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 132481 | 1.4080604475107128 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 28718 | 0.3052262583435561 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 43530 | 0.0 | 43.519394 | 1 |
TCGTGTA | 43710 | 0.0 | 43.37403 | 14 |
GTCGTGT | 45205 | 0.0 | 43.3314 | 13 |
AGAGCGT | 44775 | 0.0 | 43.32991 | 8 |
ATCGGAA | 43445 | 0.0 | 43.3159 | 2 |
CGTCGTG | 45050 | 0.0 | 43.07541 | 12 |
GATCTCG | 43690 | 0.0 | 43.0421 | 34 |
CGTGTAG | 43955 | 0.0 | 43.00737 | 15 |
GCGTCGT | 45270 | 0.0 | 42.99705 | 11 |
ATCTCGG | 43755 | 0.0 | 42.953022 | 35 |
AAGAGCG | 44195 | 0.0 | 42.938087 | 7 |
GTGGTCG | 43375 | 0.0 | 42.87062 | 41 |
GGTCGCC | 42605 | 0.0 | 42.86579 | 43 |
TCTCGGT | 44340 | 0.0 | 42.80805 | 36 |
TCGGAAG | 44310 | 0.0 | 42.72848 | 3 |
AGCGTCG | 45145 | 0.0 | 42.61906 | 10 |
TCGGTGG | 44975 | 0.0 | 42.600986 | 38 |
CTCGGTG | 44160 | 0.0 | 42.583996 | 37 |
CGGTGGT | 44690 | 0.0 | 42.570168 | 39 |
GAGCGTC | 45125 | 0.0 | 42.569702 | 9 |