Basic Statistics
Measure | Value |
---|---|
Filename | G128-M5A-B_ACAGTG_AC8WFRANXX_L001_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11650472 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 300940 | 2.5830713124755804 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCGTCGT | 40480 | 0.0 | 43.188816 | 11 |
CGTCGTG | 40675 | 0.0 | 42.906223 | 12 |
TCGTGTA | 39825 | 0.0 | 42.844242 | 14 |
AGCGTCG | 40325 | 0.0 | 42.76563 | 10 |
CGTGTAG | 39610 | 0.0 | 42.760403 | 15 |
GATCGGA | 39830 | 0.0 | 42.75693 | 1 |
TCTCGGT | 40075 | 0.0 | 42.444668 | 36 |
GTCGTGT | 41475 | 0.0 | 42.434006 | 13 |
AGAGCGT | 40795 | 0.0 | 42.41313 | 8 |
GATCTCG | 39655 | 0.0 | 42.406025 | 34 |
GAGCGTC | 40535 | 0.0 | 42.354122 | 9 |
ATCGGAA | 39910 | 0.0 | 42.329456 | 2 |
GTAGGGA | 38560 | 0.0 | 42.327126 | 18 |
GTAGATC | 39860 | 0.0 | 42.3136 | 31 |
TGTAGGG | 40795 | 0.0 | 42.089935 | 17 |
TGGTCGC | 39480 | 0.0 | 42.072067 | 42 |
ATCTCGG | 39990 | 0.0 | 42.017776 | 35 |
GTGGTCG | 39945 | 0.0 | 41.99973 | 41 |
GTCGCCG | 39615 | 0.0 | 41.983013 | 44 |
GTGTAGG | 40910 | 0.0 | 41.9286 | 16 |