FastQCFastQC Report
Tue 17 May 2016
G128-M5A-B_ACAGTG_AC8WFRANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M5A-B_ACAGTG_AC8WFRANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11650472
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3009402.5830713124755804Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT404800.043.18881611
CGTCGTG406750.042.90622312
TCGTGTA398250.042.84424214
AGCGTCG403250.042.7656310
CGTGTAG396100.042.76040315
GATCGGA398300.042.756931
TCTCGGT400750.042.44466836
GTCGTGT414750.042.43400613
AGAGCGT407950.042.413138
GATCTCG396550.042.40602534
GAGCGTC405350.042.3541229
ATCGGAA399100.042.3294562
GTAGGGA385600.042.32712618
GTAGATC398600.042.313631
TGTAGGG407950.042.08993517
TGGTCGC394800.042.07206742
ATCTCGG399900.042.01777635
GTGGTCG399450.041.9997341
GTCGCCG396150.041.98301344
GTGTAGG409100.041.928616