FastQCFastQC Report
Tue 17 May 2016
G128-M6-B_GCCAAT_AC8WFRANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M6-B_GCCAAT_AC8WFRANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11260734
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC4161333.695434063179185TruSeq Adapter, Index 6 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT546490.4853058424077862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA481150.043.6989481
ATCGGAA485150.043.1263122
ACACGTC483450.042.73647713
CGTATGC484500.042.704444
CGTCTGA484800.042.70368616
CGCCAAT483050.042.667333
ACGTCTG486400.042.63105415
TCGGAAG492100.042.5574653
ACGCCAA487250.042.51738432
CTCGTAT482950.042.5089342
GTCACGC491700.042.44953529
CACACGT488700.042.4439212
CACGTCT487200.042.43010714
TCGTATG491050.042.40357643
TATCTCG466450.042.3043839
AGTCACG494550.042.2213928
CGGAAGA494000.042.2156564
TCTCGTA473500.041.89843841
CCAATAT478200.041.46492835
GCCAATA495400.041.45153834