Basic Statistics
Measure | Value |
---|---|
Filename | G128-M6-B_GCCAAT_AC8WFRANXX_L001_001.R1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11260734 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC | 416133 | 3.695434063179185 | TruSeq Adapter, Index 6 (100% over 50bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 54649 | 0.4853058424077862 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 48115 | 0.0 | 43.698948 | 1 |
ATCGGAA | 48515 | 0.0 | 43.126312 | 2 |
ACACGTC | 48345 | 0.0 | 42.736477 | 13 |
CGTATGC | 48450 | 0.0 | 42.7044 | 44 |
CGTCTGA | 48480 | 0.0 | 42.703686 | 16 |
CGCCAAT | 48305 | 0.0 | 42.6673 | 33 |
ACGTCTG | 48640 | 0.0 | 42.631054 | 15 |
TCGGAAG | 49210 | 0.0 | 42.557465 | 3 |
ACGCCAA | 48725 | 0.0 | 42.517384 | 32 |
CTCGTAT | 48295 | 0.0 | 42.50893 | 42 |
GTCACGC | 49170 | 0.0 | 42.449535 | 29 |
CACACGT | 48870 | 0.0 | 42.44392 | 12 |
CACGTCT | 48720 | 0.0 | 42.430107 | 14 |
TCGTATG | 49105 | 0.0 | 42.403576 | 43 |
TATCTCG | 46645 | 0.0 | 42.30438 | 39 |
AGTCACG | 49455 | 0.0 | 42.22139 | 28 |
CGGAAGA | 49400 | 0.0 | 42.215656 | 4 |
TCTCGTA | 47350 | 0.0 | 41.898438 | 41 |
CCAATAT | 47820 | 0.0 | 41.464928 | 35 |
GCCAATA | 49540 | 0.0 | 41.451538 | 34 |