FastQCFastQC Report
Tue 17 May 2016
G128-M6-B_GCCAAT_AC8WFRANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M6-B_GCCAAT_AC8WFRANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11260734
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3564043.1650157085674877Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT461760.4100620794346088No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA347140.30827475367058665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA449600.043.2209051
ATCGGAA447350.043.1776542
TCGTGTA452350.043.0262414
CGTGTAG450850.043.0132415
AGAGCGT459650.042.9934438
GTCGTGT467650.042.9780913
CGTCGTG463600.042.84104512
GATCTCG449600.042.83434734
TCTCGGT454000.042.8214136
ATCTCGG449300.042.7748235
GCGTCGT466250.042.64926511
GTGGTCG449300.042.5492441
AAGAGCG456200.042.498797
GTAGGGA440800.042.3964918
CTCGGTG455350.042.3562637
TCGGTGG465500.042.3327138
GTAGATC456900.042.26922231
CGGTGGT462850.042.26799439
GGTCGCC445200.042.2640843
TGGTCGC449200.042.20280542