Basic Statistics
Measure | Value |
---|---|
Filename | G128-M6-B_GCCAAT_AC8WFRANXX_L001_001.R2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11260734 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 356404 | 3.1650157085674877 | Illumina Single End PCR Primer 1 (100% over 50bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 46176 | 0.4100620794346088 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 34714 | 0.30827475367058665 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 44960 | 0.0 | 43.220905 | 1 |
ATCGGAA | 44735 | 0.0 | 43.177654 | 2 |
TCGTGTA | 45235 | 0.0 | 43.02624 | 14 |
CGTGTAG | 45085 | 0.0 | 43.01324 | 15 |
AGAGCGT | 45965 | 0.0 | 42.993443 | 8 |
GTCGTGT | 46765 | 0.0 | 42.97809 | 13 |
CGTCGTG | 46360 | 0.0 | 42.841045 | 12 |
GATCTCG | 44960 | 0.0 | 42.834347 | 34 |
TCTCGGT | 45400 | 0.0 | 42.82141 | 36 |
ATCTCGG | 44930 | 0.0 | 42.77482 | 35 |
GCGTCGT | 46625 | 0.0 | 42.649265 | 11 |
GTGGTCG | 44930 | 0.0 | 42.54924 | 41 |
AAGAGCG | 45620 | 0.0 | 42.49879 | 7 |
GTAGGGA | 44080 | 0.0 | 42.39649 | 18 |
CTCGGTG | 45535 | 0.0 | 42.35626 | 37 |
TCGGTGG | 46550 | 0.0 | 42.33271 | 38 |
GTAGATC | 45690 | 0.0 | 42.269222 | 31 |
CGGTGGT | 46285 | 0.0 | 42.267994 | 39 |
GGTCGCC | 44520 | 0.0 | 42.26408 | 43 |
TGGTCGC | 44920 | 0.0 | 42.202805 | 42 |