G128-M7-B_CAGATC_AC8WFRANXX_L001_001.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	G128-M7-B_CAGATC_AC8WFRANXX_L001_001.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9974799
Sequences flagged as poor quality	0
Sequence length	50
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	518298	5.196074627669189	Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	204117	2.0463269485430233	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30522	0.30599112824228336	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	66055	43.526226	1
TCGTGTA	66330	43.46805	14
ATCGGAA	65830	43.377563	2
AGAGCGT	67630	43.37047	8
GTCGTGT	68770	43.31413	13
CGTGTAG	66520	43.214478	15
CGTCGTG	68355	43.181026	12
GCGTCGT	68510	43.13128	11
GATCTCG	66170	43.12454	34
AAGAGCG	66765	43.114777	7
ATCTCGG	66170	43.044746	35
TCTCGGT	66935	43.042503	36
GTGGTCG	65910	43.008804	41
GGTCGCC	64745	42.92948	43
GTAGATC	66860	42.89186	31
CGGTGGT	67470	42.87724	39
CTCGGTG	66710	42.8579	37
TGGTCGC	65295	42.84061	42
TGTAGGG	67915	42.822865	17
AGCGTCG	68320	42.816525	10