FastQCFastQC Report
Tue 17 May 2016
G128-M8-B_ACTTGA_AC8WFRANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M8-B_ACTTGA_AC8WFRANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12676090
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1411221.113292821366841TruSeq Adapter, Index 8 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA241130.19022427262665378No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224850.17738119562104718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA175900.042.623981
CGTATGC176900.041.6733344
TCGTATG178800.041.562743
CGTCTGA179250.041.14990616
ACACGTC179650.041.0950213
CTCGTAT176150.041.0889342
ACGTCTG182300.040.65451415
ATCGGAA183500.040.628422
CACGTCT183500.040.32871214
CACACGT186450.039.77322812
TCTCGTA175200.039.52866741
CGGAAGA190500.038.96234
TCGGAAG193050.038.698343
TTGAATC183050.037.4498136
GCACACG203950.036.78113611
CACTTGA202150.036.54665433
GAACTCC206000.036.4257921
TGAATCT190350.036.30138837
ATCTCGT191850.036.23571840
CTGAACT208900.036.02542519
Produced by FastQC (version 0.11.2)