FastQCFastQC Report
Tue 17 May 2016
G128-M9-B_GATCAG_AC8WFRANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M9-B_GATCAG_AC8WFRANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15465277
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1667971.0785257839222666TruSeq Adapter, Index 9 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494180.3195416415755114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA204200.042.6407661
ATCGGAA210000.041.441292
TCGTATG208500.041.33970343
CGTATGC208350.041.1371744
ACGATCA209050.041.12903232
CTCGTAT203100.041.1281942
CGTCTGA208850.040.9742916
ACACGTC209300.040.8441513
ACGTCTG210750.040.8136615
CGATCAG210950.040.643233
TCACGAT212000.040.515230
CACGTCT214450.040.0581914
CACGATC215200.039.91274331
GTCACGA216150.039.76657529
CACACGT216650.039.65141312
TCTCGTA202000.039.4680641
TCGGAAG221100.039.2612953
AGTCACG222700.038.6658728
CGGAAGA225300.038.3438764
GATCTCG208250.038.16709539
Produced by FastQC (version 0.11.2)