FastQCFastQC Report
Tue 17 May 2016
G129-M2_CGATGT_AC8VLWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M2_CGATGT_AC8VLWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14919330
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC4698033.148955080422512TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA546600.043.4208451
ATCGGAA551950.042.8335152
CGTATGC550200.042.53834544
ACACGTC558400.042.47241613
CGTCTGA558600.042.3469416
TCGTATG554250.042.3174143
ACGTCTG561950.042.2589115
CTCGTAT545850.042.25573342
TATCTCG515850.042.22466339
CACGTCT565050.042.05821214
TCACCGA551450.042.03644630
ACCGATG548400.041.99533532
CGATGTA545350.041.9602234
CACACGT565450.041.93897612
CCGATGT547450.041.9074833
CACCGAT551400.041.828831
TCTCGTA524900.041.75317841
GTCACCG563850.041.56848529
ATCTCGT528400.041.45593640
CGGAAGA567400.041.4497874
Produced by FastQC (version 0.11.2)