FastQCFastQC Report
Tue 17 May 2016
G129-M5A_ACAGTG_AC8VLWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M5A_ACAGTG_AC8VLWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5341862
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC4651838.708255660666635TruSeq Adapter, Index 5 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT706621.322797181956404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240090.4494500232315997No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC98460.18431775287343627TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTATGC86820.1625275980547607TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAGCTCGTATGC71420.13369869906785312TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACCTCGTATGC63310.11851672693903362TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA546250.043.7344131
ATCGGAA547350.043.48662
TCGGAAG549900.043.272943
ACACGTC552850.043.21671313
CGTATGC548450.043.16001544
ACGTCTG554800.043.12825815
CGTCTGA553500.043.12224216
CGGAAGA550350.043.057284
CACGTCT555750.043.05057514
CACACGT555900.043.0231312
CTCGTAT533050.042.98159842
TCGTATG551950.042.9808143
TCTCGTA503650.042.80348241
ATCTCGT504400.042.6621240
AGTGATC498650.042.6398436
GATCTCG503400.042.637239
CTGAACT560850.042.46298619
GCACACG562350.042.44360711
TCTGAAC562050.042.44277618
GAACTCC560600.042.40736421
Produced by FastQC (version 0.11.2)