FastQCFastQC Report
Tue 17 May 2016
G129-M5A_ACAGTG_AC8VLWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M5A_ACAGTG_AC8VLWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5341862
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4389638.21741557531812Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT863501.6164775503373168No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA682861.2783183092337467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA518600.043.6685451
ATCGGAA516400.043.5797042
TCGTGTA521100.043.54443414
AGAGCGT526100.043.5132188
GTCGTGT533750.043.4685913
GCGTCGT530950.043.39537411
CGTCGTG530850.043.39111712
TCTCGGT525750.043.26431736
CGTGTAG524350.043.26114315
GATCTCG520250.043.23444434
GTAGGGA513700.043.22225618
AAGAGCG520700.043.2164547
ATCTCGG521050.043.21450835
TCGGAAG525250.043.185633
GTGGTCG519800.043.13058541
GTAGATC523000.043.12949831
TGTAGGG533200.043.0979517
TCGGTGG532050.043.06585338
GAGCGTC528900.043.0528149
TGGTCGC514750.042.99079542
Produced by FastQC (version 0.11.2)