FastQCFastQC Report
Tue 17 May 2016
G129-M6_GCCAAT_AC8VLWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M6_GCCAAT_AC8VLWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17532312
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2372621.3532841532822368TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA284700.042.6798361
ATCGGAA289650.041.863372
ACGCCAA284950.041.4733932
CGCCAAT284850.041.35666333
ACACGTC296050.041.2925213
TCGTATG291900.041.25617243
CTCGTAT288600.041.13685242
CGTATGC292050.041.10077344
TATCTCG274000.040.974839
GTCACGC292650.040.92539629
CGTCTGA298850.040.83202716
ACGTCTG300100.040.6766115
CACACGT304100.040.19220412
TCTCGTA282950.040.0447541
CACGTCT305900.040.00604614
AGTCACG301300.039.91110628
ATCTCGT284850.039.73164440
TCACGCC302300.039.5534830
CACGCCA301600.039.39726631
GCACACG313100.039.0158111
Produced by FastQC (version 0.11.2)