FastQCFastQC Report
Tue 17 May 2016
G129-M6_GCCAAT_AC8VLWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M6_GCCAAT_AC8VLWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17532312
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2214661.2631876503224446Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT276900.042.46446611
CGTCGTG279300.041.9656712
CGTGTAG276450.041.9130115
AGCGTCG277900.041.80538610
TCGTGTA277950.041.67902414
GTCGTGT288200.041.1887713
TCTCGGT282350.041.17058636
GATCGGA281000.041.1177371
GTAGGGA275500.041.09932318
GTAGATC283050.041.06958831
GATCTCG280000.041.06112734
GAGCGTC283150.040.9613239
AGAGCGT284650.040.908518
TGTAGGG288600.040.6896917
GTGGTCG282650.040.5811341
GTCGCCG278450.040.54805844
TGGTCGC278200.040.5432242
ATCTCGG285000.040.54203435
GTGTAGG292350.040.14518416
ATCGGAA286800.039.989222
Produced by FastQC (version 0.11.2)