FastQCFastQC Report
Tue 17 May 2016
G131-M1-B_ATCACG_AC8WFRANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M1-B_ATCACG_AC8WFRANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13835022
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC475220.3434905994367049TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA64150.039.5670851
TCGTATG65550.038.05940643
CGTATGC66700.037.33724644
ACGTCTG68400.037.2756715
ACACGTC68250.037.0997313
CGTCTGA69550.036.56442616
ATCGGAA70350.035.8046532
ATCACGA68650.035.76341634
CTCGTAT68200.035.2579842
ACGATCT64850.034.8058937
CACACGT73500.034.6592612
CACGTCT73600.034.4328314
TCACGAT67800.034.2324635
CGGAAGA76650.033.0340084
CACGATC69100.032.76066236
CATCACG77250.032.09525333
TCTCGTA71600.031.67892641
TCGGAAG82050.031.1548483
CGATCTC74450.030.52455938
GCACACG84850.030.07490311
Produced by FastQC (version 0.11.2)