FastQCFastQC Report
Tue 17 May 2016
G131-M1-B_ATCACG_AC8WFRANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M1-B_ATCACG_AC8WFRANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13835022
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG432240.31242451222701345Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT64350.037.70853411
CGTCGTG65600.036.92306512
GATCGGA64700.036.7220951
CGTGTAG66500.035.69516415
TCGTGTA66650.035.5158114
GTCGTGT69850.035.0229413
ATCGGAA67250.034.9371072
TCTCGGT66250.034.8993236
AGAGCGT70100.034.6784828
GTAGATC68200.034.48083531
GATCTCG67150.034.43157234
AGCGTCG70050.034.3891810
GGTCGCC68350.033.42254643
ATCTCGG68800.033.3819935
GAGCGTC72550.033.0525449
GTAGGGA72150.032.4120518
GTGGTCG72300.032.07918541
TGGTCGC72150.032.02515842
GTCGCCG72450.031.80602344
CGGTGGT75000.031.21254339
Produced by FastQC (version 0.11.2)