FastQCFastQC Report
Tue 17 May 2016
G131-M10-B_CGTACG_AC8WFRANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M10-B_CGTACG_AC8WFRANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7377544
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT734520.9956158851780484TruSeq Adapter, Index 22 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320730.4347381730288562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG89650.042.4527233
GATCGGA90350.042.3660351
ACCGTAC90200.042.21853332
CGTACGT90000.042.1409634
GTACGTA90600.041.6919135
ACACGTC91350.041.58959213
ATCGGAA91750.041.4802062
CACCGTA92200.041.37431331
CGTAATC83650.041.3158838
ACGTCTG92900.041.10880715
CTCGTAT89800.040.98596644
TACGTAA91750.040.76172636
ACGTAAT87000.040.6102837
CGTCTGA94800.040.51695316
CACGTCT94550.040.32161314
GTCACCG95000.040.3169629
AATCTCG85950.040.28815541
CGGAAGA95200.039.8383334
CACACGT95650.039.7889112
TCTCGTA88300.039.56447243
Produced by FastQC (version 0.11.2)