FastQCFastQC Report
Tue 17 May 2016
G131-M10-B_CGTACG_AC8WFRANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M10-B_CGTACG_AC8WFRANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7377544
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT788641.0689736313331375No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG657150.8907435862124305Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76230.10332706927942417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT85450.042.50509311
CGTCGTG86150.042.1086512
TCGTGTA86150.041.44514
GATCGGA86000.041.4144061
AGCGTCG87050.041.29421610
GATCTCG86700.040.97909534
ATCGGAA86450.040.9698032
GTCGTGT89800.040.76540813
CGTGTAG87950.040.5467515
TCTCGGT88900.040.53414536
GAGCGTC88550.040.4704939
ATCTCGG87800.040.44062835
TGGTCGC86300.040.3395642
AGAGCGT89600.040.16818
GTGGTCG89900.039.4811341
GTAGATC90950.039.2063931
AAGAGCG90400.039.1555677
GTCGCCG89050.039.00211344
TCGGTGG93200.038.94937538
CGGTGGT93000.038.8693939
Produced by FastQC (version 0.11.2)