FastQCFastQC Report
Tue 17 May 2016
G131-M11-B_GAGTGG_AC8WFRANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M11-B_GAGTGG_AC8WFRANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6793947
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT463940.682872562885757TruSeq Adapter, Index 7 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230520.3393020287029027No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100230.14752838077777175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA57550.041.6209751
ACGAGTG57050.041.33902432
ATCGGAA58250.040.8252142
ACGTCTG57950.040.69490415
ACACGTC57850.040.68919813
CGTCTGA58500.040.31230516
CGAGTGG58650.040.24878733
CACGAGT58700.040.1014831
TCACGAG59200.040.06007430
CTCGTAT57850.039.7019544
AGTCACG60400.039.33702528
CACGTCT59950.039.30058314
TATCTCG55900.038.96200641
GTCACGA61150.038.7466329
CACACGT62250.037.95453312
CGGAAGA62600.037.8477484
TCTCGTA58400.037.4445243
TCGGAAG63750.037.303043
ATCTCGT60800.036.1111842
GGATATC61400.035.61524238
Produced by FastQC (version 0.11.2)