FastQCFastQC Report
Tue 17 May 2016
G131-M11-B_GAGTGG_AC8WFRANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M11-B_GAGTGG_AC8WFRANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6793947
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT833611.226989259704263No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG409050.6020800574393648Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84490.12436069931072467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT55350.041.37545811
CGTCGTG55750.040.9999712
AGCGTCG56700.040.1187310
GATCGGA55500.040.074481
GTCGTGT57850.039.92995513
CGTGTAG56050.039.6030315
GATCTCG55800.039.58246234
TCGTGTA56250.039.57954814
GAGCGTC57500.039.5222979
AGAGCGT57950.039.481138
ATCGGAA56350.039.3528562
TCTCGGT56800.039.31162636
ATCTCGG56950.038.8990535
TGGTCGC55950.038.8985842
GGTCGCC55500.038.85912743
GTCGCCG55950.038.70591444
CGGTGGT59250.037.8382239
GTGGTCG58750.037.71724741
GTAGATC59150.037.48834631
TCGGTGG60200.037.3856438
Produced by FastQC (version 0.11.2)