FastQCFastQC Report
Tue 17 May 2016
G131-M13-B_ATTCCT_AC8WFRANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M13-B_ATTCCT_AC8WFRANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13910849
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT13150.029.94600511
CGTCGTG16300.024.2938612
CGTGTAG18000.021.38819115
TCGTGTA18950.021.3607814
GATCGGA20150.019.9793511
AGCGTCG20050.019.7501210
TCTCGGT19850.019.61613536
GATCTCG20500.018.77953534
ATCTCGG21450.017.9478135
GTCGTGT23150.017.77060713
GTCGCCG23050.017.18695844
GTAGATC23450.016.79172331
AGAGCGT25450.016.769718
GTGGTCG24250.016.696841
GAGCGTC24700.016.4773129
TGGTCGC25400.016.20185342
GTAGGGA24650.015.79664318
TGTAGGG28150.014.61408717
CGGTGGT27400.014.37354939
GGTCGCC28450.014.15632943
Produced by FastQC (version 0.11.2)