FastQCFastQC Report
Tue 17 May 2016
G131-M14-B_AGTCAA_AC8WFRANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M14-B_AGTCAA_AC8WFRANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9412567
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570700.6063170652596683No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT460090.4888039575176463TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA60850.040.7656941
ACGTCTG63150.039.085915
ATCGGAA63850.038.450672
ACACGTC63550.038.4244813
CGTCTGA66650.037.36344516
AATCTCG60650.036.8904441
CTCGTAT65300.036.6555244
CACGTCT68750.035.67818514
CACACGT68750.035.51819212
CGGAAGA70200.035.0665934
TCTCGTA65150.034.64628243
GCACACG73000.033.35994711
AGTCAAC73900.033.073834
ATCTCGT69200.032.49140542
TCGGAAG76700.032.3816643
CAGTCAA82100.029.98513433
GTCAACA82500.029.65277335
GAACTCC85250.029.2630321
TCTGAAC89850.028.00970618
CTGAACT89850.027.93625519
Produced by FastQC (version 0.11.2)