FastQCFastQC Report
Tue 17 May 2016
G131-M2-B_CGATGT_AC8WFRANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M2-B_CGATGT_AC8WFRANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11898774
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC284220.2388649452456194TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA37350.037.400441
CGTATGC38100.035.9156844
TCGTATG39100.035.0533943
ATCGGAA41100.034.04222
TCACCGA40550.034.01652530
ACGTCTG42150.033.19417615
CGATGTA42000.032.7373934
ACACGTC42550.032.46851313
ACCGATG42350.032.3112632
CCGATGT43100.031.74873233
CGTCTGA44000.031.64851616
CACCGAT43350.031.61651831
CTCGTAT42500.031.57618742
TATCTCG41450.030.72981839
GTCACCG45250.030.62917129
CACGTCT47400.029.33195714
CACACGT47900.029.02577812
CGGAAGA49600.028.5188244
TCTCGTA45300.028.36177341
GATGTAT49450.026.29265635
Produced by FastQC (version 0.11.2)