FastQCFastQC Report
Tue 17 May 2016
G131-M2-B_CGATGT_AC8WFRANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M2-B_CGATGT_AC8WFRANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11898774
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG251750.21157641955381287Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT37600.035.16355511
CGTCGTG39300.033.69846312
GATCGGA40000.032.7784771
TCGTGTA40300.032.48001514
AGCGTCG41100.032.06217610
GATCTCG40500.031.72158234
ATCGGAA41950.031.1499212
TCTCGGT42250.031.03248436
CGTGTAG42700.030.55140115
GTCGTGT43850.030.50308413
GTAGATC42200.030.49467531
GTCGCCG42100.029.80199644
GTAGGGA42450.029.59133318
AGAGCGT45100.029.5599758
ATCTCGG43900.029.41511735
GAGCGTC45350.028.8634099
GGTCGCC44900.027.94199243
TGGTCGC45650.027.62478342
TGTAGGG48850.027.29055617
GTGTAGG50100.026.91702716
Produced by FastQC (version 0.11.2)