FastQCFastQC Report
Tue 17 May 2016
G131-M4-B_ATGTCA_AC8WFRANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M4-B_ATGTCA_AC8WFRANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13099419
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT829400.6331578522681044TruSeq Adapter, Index 15 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA234410.17894686779619767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA104800.041.2932361
ATCGGAA107500.039.8430672
CTCGTAT107350.038.5693844
ACACGTC110150.038.56497613
ACGTCTG111050.038.49014715
CGTCTGA112400.038.34116
AATCTCG104100.037.82951441
CACACGT115900.036.74660512
CGGAAGA118750.036.2907644
CACGTCT117950.036.23849514
TCTCGTA110200.035.9947643
TCGGAAG124000.034.8961873
GCACACG122550.034.64489711
ATCTCGT116100.034.27926642
ACATGTC136400.031.62945232
GAATCTC127100.031.17429440
TCTGAAC140550.030.78710218
GAACTCC140400.030.64763821
CACATGT142900.030.12916231
ATGTCAG144650.029.83967834
Produced by FastQC (version 0.11.2)