FastQCFastQC Report
Tue 17 May 2016
G131-M4-B_ATGTCA_AC8WFRANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M4-B_ATGTCA_AC8WFRANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13099419
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG745980.5694756385760315Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285840.2182081510637991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT101600.040.7939311
CGTCGTG102800.040.2110412
TCGTGTA103000.039.42827214
GATCTCG103400.039.01954334
GATCGGA103850.038.8507581
AGCGTCG105300.038.79643610
TCTCGGT106750.038.43388736
ATCGGAA105600.038.0194322
AGAGCGT109100.037.9694148
GTAGATC106900.037.94620531
ATCTCGG106700.037.91583635
GTCGCCG104350.037.90062344
GTCGTGT109950.037.83652513
CGTGTAG106750.037.775315
TGGTCGC105050.037.6648542
GGTCGCC105500.037.29845443
GAGCGTC110350.037.0409169
GTAGGGA107850.036.4518318
TGTAGGG116850.035.41386817
GTGTAGG117500.035.31157716
Produced by FastQC (version 0.11.2)