FastQCFastQC Report
Tue 17 May 2016
G131-M5-B_CCGTCC_AC8WFRANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M5-B_CCGTCC_AC8WFRANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10190693
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG286050.2806972989962508Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT43500.037.42395411
AGCGTCG44200.036.2837810
CGTCGTG44950.035.72731812
GATCTCG45000.035.24746334
TCGTGTA45400.035.13073714
GATCGGA45750.034.8140641
TCTCGGT46150.034.6551536
GTCGTGT47650.034.25691613
ATCGGAA47300.033.3476522
CGTGTAG47750.033.30964315
GAGCGTC48550.033.1234329
GTAGATC48950.032.98678231
ATCTCGG48450.032.873835
GTCGCCG48700.032.7193344
AGAGCGT50650.032.141018
GTAGGGA48900.032.12155518
TGGTCGC50050.031.56938642
TGTAGGG52800.030.87374917
GTGTAGG52500.030.7568516
GGTCGCC51100.030.66501843
Produced by FastQC (version 0.11.2)