FastQCFastQC Report
Tue 17 May 2016
G131-M6-B_GTCCGC_AC8WFRANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M6-B_GTCCGC_AC8WFRANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11368494
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT514700.45274246527288486TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG64250.040.5755632
GATCGGA65200.040.4524271
ATCGGAA67550.038.884742
CGTCCGC66950.038.87348633
ACACGTC70350.037.3996313
GTCCGCA69600.037.1716434
AGTCACG71300.037.05689228
ACGTCTG71550.037.049115
CGTCTGA72700.036.7051216
GTCACGT72250.036.53919229
CTCGTAT70300.036.45775644
TCACGTC73750.035.8855130
CGCACAT69350.035.560937
TCCGCAC75100.034.74228335
CACGTCT76650.034.52659214
CACACGT76550.034.4567412
TCTCGTA73600.033.47800443
CGGAAGA79550.033.4615024
CACGTCC79450.033.33880631
CCGCACA78400.033.0273736
Produced by FastQC (version 0.11.2)