FastQCFastQC Report
Tue 17 May 2016
G131-M7-B_GTGAAA_AC8WFRANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M7-B_GTGAAA_AC8WFRANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13289287
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA883300.6646707231170491No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT700420.5270561167051325TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA89250.040.7092481
ATCGGAA91600.039.4826852
AACGATC81750.038.88602438
TGAAACG91400.038.87225735
GAAACGA92400.038.21347436
ACACGTC93250.038.19428313
ACGTCTG93950.038.12044515
AAACGAT86250.038.0815137
CTCGTAT90250.038.07607744
GTCACGT95300.037.7665329
AGTCACG95450.037.7071828
CGTCTGA96550.037.5040316
CACGTGA96050.037.28839531
ACGATCT85600.037.1627639
CACGTCT99950.035.87609514
TCACGTG100150.035.80579830
CACACGT100500.035.72353712
CGGAAGA101550.035.5274734
TCTCGTA90300.035.39942643
TCGGAAG104650.034.4330143
Produced by FastQC (version 0.11.2)