FastQCFastQC Report
Tue 17 May 2016
G131-M7-B_GTGAAA_AC8WFRANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M7-B_GTGAAA_AC8WFRANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13289287
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT979180.7368190633553177No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG654090.4921934487531197Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT85950.040.23618711
CGTCGTG88050.039.10165812
GATCGGA88350.038.2964321
GATCTCG87450.038.26189834
AGCGTCG89500.038.0012810
TCTCGGT89200.037.88117636
TCGTGTA90250.037.7094314
ATCGGAA89200.037.6602062
GAGCGTC90950.037.4196249
GTCGTGT93900.037.22789413
GTCGCCG88100.037.14864744
ATCTCGG90800.037.01984835
CGTGTAG93600.036.52431515
AGAGCGT94000.036.4161078
GTAGATC93250.036.3057531
TGGTCGC91300.035.69834542
GGTCGCC92150.035.3478443
GTAGGGA93150.035.28387518
GTGGTCG94650.034.87452741
GTGTAGG101000.034.30568316
Produced by FastQC (version 0.11.2)