FastQCFastQC Report
Tue 17 May 2016
G131-M9-B_GTTTCG_AC8WFRANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M9-B_GTTTCG_AC8WFRANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10274427
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT498180.4848737550035637TruSeq Adapter, Index 21 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA324290.3156283070579021No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181670.17681764637580277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG64050.040.84005433
GATCGGA67700.039.6432841
ATCGGAA69250.038.5016142
ACGTCTG69150.038.4300415
ACGTTTC69050.037.9468632
ACACGTC70250.037.7969713
GTCACGT69400.037.72323229
CGTCTGA70750.037.68532616
AGTCACG70500.037.35308528
CTCGTAT68200.037.225744
TCGGAAT66150.036.88255737
CGGAATC65500.036.6439938
GTTTCGG73000.035.832634
AATCTCG67000.035.8239541
CGGAAGA74400.035.718244
CACACGT74850.035.67984412
TTTCGGA72850.035.60439335
TTCGGAA72800.035.50796536
CACGTCT74900.035.47980514
TCTCGTA69500.034.9150143
Produced by FastQC (version 0.11.2)