FastQCFastQC Report
Tue 17 May 2016
G131-M9-B_GTTTCG_AC8WFRANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M9-B_GTTTCG_AC8WFRANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10274427
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT715150.6960485484981304No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG453290.44118275403582113Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129630.12616761985850888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT63800.039.34337211
CGTCGTG66100.038.04113812
GATCTCG65900.037.72205734
TCTCGGT66800.037.57608836
ATCTCGG66950.037.2290335
TCGTGTA66800.037.14832314
CGTGTAG66850.036.98890315
GTAGATC67900.036.7715231
GATCGGA67500.036.6977231
GTCGTGT70100.036.43570313
AGAGCGT70200.036.0385488
AGCGTCG70050.035.77026710
GTCGCCG68900.035.48935344
ATCGGAA69650.035.4701582
GAGCGTC70750.035.4163559
GTAGGGA68600.035.2439318
TGGTCGC69600.035.22385442
GTGTAGG72250.035.076916
GTGGTCG70300.034.9651641
GGTCGCC69900.034.66541743
Produced by FastQC (version 0.11.2)