FastQCFastQC Report
Sun 24 Apr 2016
G129-M13_CTTGTA_AC8VLWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M13_CTTGTA_AC8VLWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15304942
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA7250.012.7581681
AATCCGA16600.011.79448924
CTCGAAT23300.011.6258911
ATCCGAA18950.010.44841525
TACGTCA43650.010.33154932
CGAATGT18600.010.289723
ATACGTC44150.010.264873531
AAATCCG19050.010.16213423
CTCGTTT26450.010.1580711
CATACGT46200.010.09510930
TCATACG46950.010.07441729
ATTGGAC60350.09.9148476
AATTGGA80400.09.3302385
GTCGAAT13950.09.31441
AACGTGC12450.09.18815619
ACGTCAC50250.09.14968333
TAAGGCG15700.09.1076889
ACGTGCC12850.08.90214420
GAATTGG81350.08.8426934
CCGGGAT16500.08.8092121
Produced by FastQC (version 0.11.2)