FastQCFastQC Report
Sun 24 Apr 2016
G129-M14_AGTCAA_AC8VLWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M14_AGTCAA_AC8VLWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13658654
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9450.014.91447451
ATCGGAA11600.012.3269442
TCGACCG3653.6379788E-1212.05465327
CTCGAAT19250.010.9824771
CGTCTGA13900.010.12893216
CTCGTAT9950.09.9504742
TAAGGCG17600.09.8744519
CCGGAAT10050.09.8606661
CTCGGTT18950.09.7617981
ACTACGT7600.09.55209912
ATACGTC35950.09.42408931
TACGTCA35850.09.38863432
CATACGT38050.09.36651330
CGATATA4754.292815E-109.2626424
TCATACG38750.09.19731129
CGACCGT4602.3555913E-99.0868528
ACGTCTG16800.09.03521215
TCGGTTT23400.08.931162
ATTGGAC50050.08.8346736
AATTGGA66350.08.8194045
Produced by FastQC (version 0.11.2)