FastQCFastQC Report
Sun 24 Apr 2016
G129-M14_AGTCAA_AC8VLWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M14_AGTCAA_AC8VLWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13658654
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC25450.023.0799051
ACGATCA27000.020.9413052
CGATCAC29500.019.766793
GCGTCGT9600.014.20746811
CGTCGTG10150.013.00408612
AATGTAG58950.011.1235845
TAATGTA56950.010.8187384
TCACAAG58450.010.5787446
CGTGTAG10750.010.43653615
TCGTGTA10350.010.41478614
AGCGTCG13100.010.24376310
CCGGAAT23950.010.1041011
CACGATA17800.09.76380842
ACGATAC18350.09.71093643
TATACGC2556.907317E-59.4921557
CGATACA17750.09.41993744
GATCTCG10050.09.41277534
CTTTAAT82650.09.34275151
ACGCTAA7550.09.32420826
ATGTAGA70200.09.0901956
Produced by FastQC (version 0.11.2)