FastQCFastQC Report
Sun 24 Apr 2016
G129-M15_AGTTCC_AC8VLWANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG129-M15_AGTTCC_AC8VLWANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12698340
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10100.014.3912541
CTCGAAT19600.011.3485771
CGTCTGA14850.011.11036616
CTCGTAT11150.011.05037142
TACGTCA28050.010.8228932
ATACGTC28400.010.53516931
AATCCGA16150.010.488461524
ATCCGAA15300.010.352925
ATCGGAA13450.010.1406242
CATACGT29750.010.13105230
ACGTCTG15500.010.07674415
CGGGGTT22550.010.0592761
CGGGTTA5051.8189894E-1210.0302681
TCATACG30350.010.00325529
CGAATGT17200.09.9761143
CGGGCTA7000.09.7530341
CGCGTAT2751.4858249E-59.6100271
GTCGGTT22150.09.5449491
CGGGATT11900.09.4384191
AAATCCG17800.09.39262923
Produced by FastQC (version 0.11.2)