FastQCFastQC Report
Sun 24 Apr 2016
G130-M10_ATGTCA_AC8VLWANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M10_ATGTCA_AC8VLWANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10432052
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTT20600.011.1063672
CTCGAAT15300.010.7929491
GTCGGTT18000.010.6418481
CGGGCTA5101.8189894E-129.9295141
CGGTTTG22300.09.7664393
CTCGGTT14600.09.6515581
CGGGATT7900.09.4759371
AACGTGC9100.09.42816219
CGAATGT13700.09.1529313
TAAGGCG15950.09.1030539
CGGGTTT11200.08.8463641
ACGTGCC9550.08.75354620
CTCGTTT17250.08.6794081
CTCGTAT6403.6379788E-128.6006321
GTCCGTT9000.08.5624071
TTCGATT12150.08.5099582
ACCGGTA8050.08.47168227
GCCGGAT7900.08.361121
GTCGGAA16400.08.3237331
GCCGTTA2650.00105001548.3085351
Produced by FastQC (version 0.11.2)