FastQCFastQC Report
Sun 24 Apr 2016
G130-M10_ATGTCA_AC8VLWANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG130-M10_ATGTCA_AC8VLWANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10432052
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG23800.011.9241913
CGATACA12050.011.1363944
CCGGAAT16100.011.06996251
CGACGAT5250.010.89685251
CACGATA12450.010.77859442
ACGATAC12750.010.69752143
CGATCAC6300.010.4760343
CACTAGC30100.09.64769654
ACTAGCC31050.09.2108135
CCCACTA31800.08.856362
CGAATAA7950.08.8556820
TCACGAT14950.08.82900341
GGAAGAT67200.08.8078521
CGGAATT18300.08.7769612
CGACAAC14500.08.6495561
AATCGAA8500.08.5410495
ACGCAAC6201.2732926E-118.51575710
TACGCAA6503.6379788E-128.461259
TATAACG2608.765605E-48.4612097
TTCACGA17100.08.36200740
Produced by FastQC (version 0.11.2)